I have experienced pretty significant changes in tone in the first few weeks of owning brad new guitars (luthier built), particularly when red spruce is involved. Over the first few weeks, they lose the stiffness (harsh respond to pick attack with little sustain or overtone) and start to bloom and sustain. The difference is substantial and notable. However, after this initial break-in, I've not been able to easily track in my mind's ear how things change over the long term. It's very slow, for sure.
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