I have buffalo horn on my old D-18, I think I can hear a difference, I doubt anyone else does. And I have ebony on several other guitars.
Beyond what I can and can't hear, I like the idea of natural ingredients on my guitars when I have a choice (bone, ivory, wood, horn, etc.).
But this is one of those instances where half the room will say they do hear a difference in pin material, the other half will say they're nuts, there just isn't any. So, if you hear a difference, then there is one.
Different strings and picks, however, do make a substantial difference.
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